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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
25/01/2016 |
Data da última atualização: |
03/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ROSSONI, R. D.; BARBOSA, J. O.; VILELA, S. F. G.; SANTOS, J. D. dos; BARROS, P. P. de; PRATA, M. C. de A.; ANBINDER, A. L.; FUCHS, B. B.; JORGE, A. O. C.; MYLONAKIS, E.; JUNQUEIRA, J. C. |
Afiliação: |
Rodnei Dennis Rossoni; Júnia Oliveira Barbosa; Simone Furgeri Godinho Vilela; Jéssica Diane dos Santos; Patrícia Pimentel de Barros; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Ana Lia Anbinder; Beth Burgwyn Fuchs; Antonio Olavo Cardoso Jorge; Eleftherios Mylonakis; Juliana Campos Junqueira. |
Título: |
Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
PLoS One, v. 10, n. 7, 2015. |
Páginas: |
23 p. |
Idioma: |
Português |
Conteúdo: |
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis. MenosIn this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans ha... Mostrar Tudo |
Palavras-Chave: |
Animal model. |
Thesaurus Nal: |
Galleria mellonella; infection. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137696/1/Cnpgl-2015-PlosOne-Competitive.pdf
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Marc: |
LEADER 02761naa a2200289 a 4500 001 2035034 005 2024-02-03 008 2015 bl uuuu u00u1 u #d 100 1 $aROSSONI, R. D. 245 $aCompetitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.$h[electronic resource] 260 $c2015 300 $a23 p. 520 $aIn this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis. 650 $aGalleria mellonella 650 $ainfection 653 $aAnimal model 700 1 $aBARBOSA, J. O. 700 1 $aVILELA, S. F. G. 700 1 $aSANTOS, J. D. dos 700 1 $aBARROS, P. P. de 700 1 $aPRATA, M. C. de A. 700 1 $aANBINDER, A. L. 700 1 $aFUCHS, B. B. 700 1 $aJORGE, A. O. C. 700 1 $aMYLONAKIS, E. 700 1 $aJUNQUEIRA, J. C. 773 $tPLoS One$gv. 10, n. 7, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registros recuperados : 13 | |
2. | | LIMA, J. de A.; JUNQUEIRA, J. G. O.; BUSO, J. A.; SOUZA, A. F.; MAKISHIMA, N.; CABREIRA, J. Efeito do nitrogenio e fosforo na producao de bulbos de cebola. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 22., 1982, Vitoria, ES. Resumos... Vitoria: SOB, 1982. p.316.Biblioteca(s): Embrapa Hortaliças. |
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3. | | JUNQUEIRA, J. R. de J.; LIMA JÚNIOR, F. A. de; FERNANDES, G. S.; PAES, M. C. D.; PEREIRA, J. Proximate composition and technological characteristics of dry pasta incorporated with micronized corn pericarp. Revista Caatinga, Mossoró, v. 30, n. 2, p. 496-502, abr./jun. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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5. | | BATISTA, G. O.; JUNQUEIRA, J.; SILVA, V. S.; DALMEDICO, G.; MOURA, M. N.; TEIXEIRA, I.; SABAINÍ, R. M.; SILVA, R. D. da; MOREIRA JÚNIOR, J. P. R. Sistema integrado de manejo de fauna. In: OLIVEIRA JÚNIOR, J. M. B.; CALVÃO, L. B. (Org.). Tópicos integrados de zoologia 2. Ponta Grossa, PR: Atena, 2020. p. 1-9.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Suínos e Aves. |
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6. | | JORJÃO, A. L.; OLIVEIRA, L. D.; SCORZONI, L.; FIGUEIREDO-GODOI, L. M. A.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C. From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies. Virulence, v. 9, n. 1, p. 383-389, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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7. | | VILELA, S. F. G.; BARBOSA, J. O.; ROSSONI, R. D.; SANTOS, J. D.; PRATA, M. C. de A.; ANBINDER, A. L.; JORGE, A. O. C.; JUNQUEIRA, J. C. Lactobacillus acidophilus ATCC 4356 inhibits biofilm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella. Virulence, v. 6, n. 1, p. 29-39, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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8. | | ROSSONI, R. D.; BARBOSA, J. O.; VILELA, S. F. G.; SANTOS, J. D.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C. Interaction between Candida albicans and krusei: in vivo study using galleria mellonella and immunosuppressed mice as a model hosts. In: CONGRESS OF EUROPEAN MICROBIOLOGISTS, 5., 2013, Leipzig, Germany. Abstracts... Leipzig: [s.n.], 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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9. | | LIMA, J. A.; BUSO, J. A.; SOUZA, A. F.; MAKISHIMA, N.; JUNQUEIRA, J. G. O.; FERREIRA, P. F.; C. FILHO, J. Producao de cebola em funcao de niveis de aplicacao de nitrogenio e fosforo. Horticultura Brasileira, Brasilia, v.2, n.2, p.12-14, nov. 1984.Biblioteca(s): Embrapa Hortaliças. |
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10. | | ROSSONI, R. D.; BARBOSA, J. O.; VILELA, S. F. G.; SANTOS, J. D. dos; BARROS, P. P. de; PRATA, M. C. de A.; ANBINDER, A. L.; FUCHS, B. B.; JORGE, A. O. C.; MYLONAKIS, E.; JUNQUEIRA, J. C. Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis. PLoS One, v. 10, n. 7, 2015. 23 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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11. | | COSTA, O. A. D.; SILVA, J. L. S. da; FERREIRA, O. G. L.; COELHO, R. A. T.; BRONDANI, W. C.; KRÖNING, A. B.; WIEBUSH, A. T.; JUNQUEIRA, J. F. Rendimento de sorgo corte/pastejo no outono em sistema de integração lavoura-pecuária. In: REUNIÃO TÉCNICA ANUAL DO MILHO, 58.; REUNIÃO TÉCNICA ANUAL DO SORGO, 41., 2013, Pelotas. Resumos... Brasília, DF: Embrapa, 2014.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Clima Temperado. |
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12. | | PEDRINHA, V. F.; SANTOS, L. M.; GONÇALVES, C. P.; GARCIA, M. T.; LAMEIRA, O. A.; QUEIROGA, C. L.; MARCUCCI, M. C.; SHAHBAZI, M.-A.; SHARMA, P. K.; JUNQUEIRA, J. C.; SIPERT, C. R.; ANDRADE, F. B. de. Effects of natural antimicrobial compounds propolis and copaiba on periodontal ligament fibroblasts, molecular docking, and in vivo study in Galleria mellonella. Biomedicine & Pharmacotherapy, v. 171, 116139, 2024.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental. |
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13. | | BARBOSA, J. O.; ROSSONI, R. D.; VILELA, S. F. G.; ALVARENGA, J. A. de; VELLOSO, M. dos S.; PRATA, M. C. de A.; JORGE, A. O. C.; JUNQUEIRA, J. C. Streptococcus mutans can modulate biofilm formation and attenuate the virulence of Candida albicans. Plos One, v. 11, n. 3, e0150457, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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